Diatec AS
Gaustadalléen 21
N-0349 Oslo, Norway

Tel: (+47) 22 95 86 25
Fax: (+47) 22 95 86 49
diatec@diatec.com

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Mice Friendly

Production of mAbs at Diatec's scale in vivo would require over 3000 mice annually.
We produce all our antibodies in vitro !


Since Köhler & Milsteins discovery of hybridomas producing monoclonal antibodies 1 in 1975, most production has been done in animals. The hollow fibre technology has developed over the same period 2 , and has the last decade given us an excellent alternative to using production animals. These systems have now been valued such a good alternative that several European countries have banned the use of animals/ascites for antibody production 3 . Also the US is changing in this direction 4 .


TECNOMOUSE 		Production
For production in the scale 100 to 1500 mg - we cultivate our clones and produce our mAbs in the TECNOMOUSE system from Integra Biosciences . This is a very flexible system and we have been using several variants for two years, with great success. In vitro produced mAbs are more consistent and have higher specific activity than those produced in ascites.

For smaller production scale - up to 100 mg - we use the CELLine CL1000 system, also from Integra Biosciences. The CELLine range is based on the same membrane technology as the Tecnomouse.


CELLine CL1000

For more information please contact diatec@diatec.com .

Save mice - save money - buy your mAbs from Diatec! Place your order here .


References:
  1. Köhler G., Milstein C., (1975). Continous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495-497.
  2. Knazek R.A., Gullino P.M., Kohler P.O. and Dedrick R.L. (1972). Cell culture on artificial capillaries: an approach to tissue growth in vitro. Science 178: 65-67.
  3. Monoclonal antibody production, The Report and Recommendations of ECVAM Workshop 23, Alternatives to Laboratory Animals (ATLA) journal, volume 25, April 1997 http://www.nal.usda.gov/awic/pubs/antibody/ecvam.htm
  4. Alternatives in monoclonal antibody production, A Report of the CAAT Technical Workshop of September 24-25, 1997, http://www.jhsph.edu/~caat/pubs/tech_reports/techreport08.htm
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