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MoFlo® MLS Flow Cytometer
MoFlo is a revolutionary flow cytometer, designed to meet the most exacting standards of high speed
performance and modularity. The MoFlo incorporates proprietary patented parallel processing parts
developed at the Lawrence Livermore National Laboratory (LLNL) and was used successfully as a
chromosome sorter for the Human Genome Project.
LLNL scientists were able to sort their allocated chromosomes for the compilation
of chromosome-specific DNA libraries within the allocated twelve-month
timeframe, using the sorter just one week per month. Other
groups, relying on conventional flow cytometers, worked around the
clock to achieve similar results.
Cytomation was selected by LLNL to bring MoFlo from prototype
to commercial instrument, adding at the same time new features and
functionality, as well as a new level of engineering quality.
High Speed Sorting
The MoFlo (modular flow) cytometer was designed, first and foremost, as a high-speed sorter. It offers the sorting precision required for the isolation of rare cell populations, with high purity and yield. The MoFlo is designed to sort at up to 70,000 events per second, approximately 5 to 10 times faster than existing instruments. Sorted cells must be viable, purity must be greater than 99 percent at any speed, and yield must be solely determined by Poisson statistics. To illustrate this with an example, the MoFlo can sort 5x106 cells from a 0.5 % population in 5.6 hours. Other instruments may take as long as 10 or more hours to complete this same sort. The high sort speed of the MoFlo provides the user with unparalleled performance.
| MOFLO CYTOMETER TECHNICAL SPECIFICATIONS |
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| Sorting Method: | Jet-in-air |
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| Sorting Rate: | 70,000 events/second |
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| Sorting Purity: | > 99% for 70,000 events/second |
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| Sorting Modes: | 15 modes (3 x 5 matrix of purity, enrich, single vs. 0.5, 1, 1-2, 2, 3 drops) plus mixed mode |
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| Compensation: | 8 x 8 matrix, ratios and time |
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| Acquisition Speed: | 150,000 events/second |
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| Fluorescence Sensitivity: | < 200 with Spherotech Rainbow calibration particles |
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| Fluorescence Resolution (CV): | < 2% DAPI stained trout erythrocytes |
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| Optical Parameters: | 2 scatter and 10 fluorescence standard,16 fluorescence available |
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| Beam Geometry: | Spherical and elliptical |
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| Number of Lasers: | 1-3 |
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| Accepted Lasers: | Argon, Krypton, mixed gas ion, dye heads, HeNe, multi-line UV, Cytomation’s 635 nm solid-state diode laser |
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| Fluidic System Rating: | up to 100 psi (689 kPa) |
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| CytoNozzle Orifice: | 50, 70, 100, 150, 200, 400 mm diameter |
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| Sample Flow Rate: | 30 ml/min at 0.5 psi (3.5 kPa) differential |
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| Optical Bench Sizes (feet): | 4 x 6, 4 x 5, 4 x 4, 3 x 4, 3 x 2.5, 2.5 x 2.5 |
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| Optical Bench Grid: | 1 inch center, 1/4-20 holes |
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| Benchtop System Dimensions: | 125.0 cm wide x 76.25 cm deep x 74.0 cm tall |
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| SUMMIT WORKSTATION SPECIFICATIONS |
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| Platform: | Windows NT operating system |
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| Motherboard: | Dual Intel 400 MHz Pentium® II with 128 MB RAM expandable to 1 GB |
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| Storage Space: | Two 8.4 GB hard disks, CD-ROM drive, optional DVD, CD-RW |
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| Monitor: | Single or dual-monitor 16 MB video card, one or two 21-inch or larger traditional tube-type or LCD flat screen monitors |
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| Miscellaneous: | Ethernet LAN card, 3.5” disk drive, sound card and speakers |
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For more information about our products, please contact Cytomation
at information@cytomation.com.
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