MoFlo® Accessories
MoFlo 4Way™ Sorting
4Way Sorting upgrades the standard two stream capability of the MoFlo cytometer to three and four stream capability. This means researchers can sort more subsets faster and waste fewer cells than has ever before been possible.
Simultaneous Subsets
As cells of interest become more specifically defined, researchers find themselves sorting a given sample multiple times to extract the desired cells. This wasteful and inefficient strategy is no longer necessary. With 4Way Sorting, a researcher can define up to four unique populations of interest based on as many fluorescence parameters as there are photo-multiplier tubes (PMTs) on the system (Figure 1). The 4Way Sorting accessory adds the sort logic and counters for the additional sorted streams, allowing three or four subsets to be simultaneously sorted into a variety of standard tubes or cuvettes.
Digital Stream Control
The Cytomation Sort Unit (CSU) controls droplet formation during a sort. This all-digital computer-controlled device allows the operator to manipulate both droplet shape, by virtue of the total programmability of the droplet formation waveform, and droplet charging. The dedicated processor in the CSU is actually a computer in itself. This means that unlike analog modules that have built-in obsolescence, the CSU can be re-programmed to meet future needs.
Simple Operation
4Way Sorting is a feature that may be routinely used by any operator. The difference between normal bi-directional sorting and 4Way Sorting is just a couple of simple steps; the user interface is essentially the same.
Future Automation
The 4Way Sorting Accessory is another module in Cytomation's continuing plan to automate the MoFlo's sort process. Cytomation is unique in employing microprocessor-control for these modules. These modules are programmable and will be able to communicate with each other to coordinate the sort process. Cytomation is designing the power and flexibility necessary for a fully integrated, automated sort.
| 4WAY SORTING TECHNICAL SPECIFICATIONS |
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| 4Way Sorting Accessory includes: | Lookup Table Module (qty 2) |
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| CSU Counter/Abort Module |
| 4Way Sorting base |
| 4Way Sorting cover |
| 1.5 ml micro-tube holder |
| Cytomation 7 ml 4Way cuvette holder |
| Cytomation 7 ml 4Way cuvettes (qty 8) |
| 1.5 ml micro-tubes (qty 1000) |
| Collection Tube Sizes: | 1.5 ml micro-tubes, Cytomation 7 ml 4Way cuvettes |
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| Stream Separations: | 13 mm |
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| Maximum Cells per Second: | 70,000 |
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| Maximum Droplet Formation Rate: | 200,000 drops/second |
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| Chargin Voltages: | 200 volts |
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For more information about our products, please contact Cytomation
at information@cytomation.com.
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