Flow Diagram of ABSOLUTE-S^TM Cell Proliferation Assay

The following is an outline of the steps employed in the ABSOLUTE-S^TM Cell Proliferation Assay

RESEARCHER'S TEST CELLS		GROW CELLS IN BrdUrd
		UV IRRADIATE CELLS		FIXED CONTROL CELLS FROM KIT
		FIX CELLS
		WASH CELLS
		LABEL DNA IN CELLS
		RINSE CELLS
		STAIN CELLS WITH FLUORESCEIN~PRB-1
		PROPIDIUM IODIDE RNASE/A TREATMENT		NOTE: Optional when using other cell markers.
		FLOW CYTOMETRY ANALYSIS