COULTER™ CD95-RD1
CD95, also known as the Fas antigen, can trigger apoptosis or programmed cell death. This antigen is found on 80-90% of activated T cells, B cells and a majority of activated NK cells. COULTER CD95-RD1 is a ready-to-use, liquid antibody for fundamental research and is not for clinical diagnostic use.
Light Scatter Histogram (CD95-RD1)
The light scatter pattern of lysed whole blood cellular populations is readily discernable on
a FS vs. LSS dot density histogram. A gating region is set around the lymphocyte population.
Example Research Areas:Apoptosis in Peripheral Blood
Iwai, K. et al. Blood, 1994, 84(4):1201.Evaluation of Activated T Cells in
Acute Viral Infection
Akbar, A. et al. J Exp Med, 1993, 178:427.Regulation of the Human Immune Response
Daniel, P. & Krammer, P. J Immunol, 1994, 152:5624.Apoptosis and Autoimmune Disease
Nakajima, T. et al. Arth Rheum, 1995, 38(4):485.
Amasaki, Y. et al., Clin Exp Immunol, 1995, 99:245.
Rieux-Laucat, F., et al.Science, 1995, 268:1347.
Log Fluorescence Histogram (CD95-RD1)
Single parameter histogram of a lysed whole blood sample stained with CD95-RD1 and gated on the lymphocyte region. The analysis region is based on an isotypic control.
Molecular Orientation
CD95 or Fas antigen is a 48 kD transmembrane glycoprotein which contains 3 cysteine-rich repeats. There is some homology in the cytoplasmic sequence between CD95 and TNF-R1 (CD120a).
Cell Type
CD95 antigen is expressed on a variety of cells, primarily activated T & NK cells. It is not found on erythrocytes or platelets.
COULTER (TM)
CD95-RD1Specificity
CD95 is found on the surface of 30-40% of resting T cells, B cells, 5% of NK cells and thymocytes. Activated NK cells and 80-90% of activated T cells express CD95. It is not found on platelets or erythrocytes, but is expressed variably on granulocytes and a subset of monocytes. It is a member of the nerve growth factor receptor/tumor necrosis factor receptor (NGFR/TNFR) superfamily.
Product Description
COULTER CD95-RD1 is in liquid form and is directly conjugated.
Concentration: 1µg/10 µL
Vial Contents: 0.5 mL
Isotype: IgM
Part Number: 6607003
Chromosome 10
The CD95 gene is located on human Chromosome 10, loci q24.1
For Research Use Only. Not for Use in Diagnostic Procedures.
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