COULTER™ CD28-FITC, CD28-RD1
CD28 antigen is a co-stimulatory molecule for T cell activation. It is found on both resting and activated T cells. Ligation of CD28 by CD 80 (B7) on antigen presenting cells leads to an increased cytokine production and T cell proliferation. COULTER CD28-FITC and CD28-RD1 are ready-to-use, liquid antibodies for fundamental research and are not for clinical diagnostic use.
Log Fluorescence Histogram (CD28-FITC)
Single parameter histogram of a lysed whole blood sample stained with CD28-FITC and gated on the lymphocyte region. The analysis region is based on an isotypic control.
Example Research Areas:Studies of T Cell Development and Subsets
Mauri, D. et al. J Immunol, 1995, 155:118.
June, C.H. et al. Immunology Today, 1990, 11(6):211.Cell Adhesion Studies
Linsley, P.S. et al. PNAS, 1990, 87:5031.
June, C.H. et al. Immunology Today, 1994, 15(7):321.T Cell Cytokine Regulation
Cerdan, C. et al. J Immunol, 1995, 154:1007.
Olive, D. et al. Fund Clin Immunol, 1994, 2(4):1122.
Log Fluorescence Histogram (CD28-RD1)
Single parameter histogram of a lysed whole blood sample stained with CD28-RD1 and gated on the lymphocyte region. The analysis region is based on an isotypic control.
Molecular Orientation
CD28 antigen has a molecular weight of 88 kD. It is a disulfide-linked homodimeric glycoprotein.
Cell Type
CD28 is a T cell activation marker found on the surface of most T lineage cells, and may be found on plasma cells.
Ontogeny
COULTER (TM)
CD28-FITC, CD28-RD1Specificity
CD28 is a glycoprotein expressed on the surface of 95% of CD4 lymphocytes, 50% of CD8 lymphocytes and 5% of immature CD3 thymocytes. The density of CD28 increases on CD4+CD8+ thymocytes as they mature. CD28 antigen density is further enhanced by activation.
Product Description
COULTER CD28-FITC and CD28-RD1 are in liquid form and are directly conjugated.
Concentration: 1µg/10 µL
Vial Contents: 0.5 mL
Isotype: IgG1
Part Number: FITC 6607004
Part Number: RD1 6607005
Chromosome 2
The CD28 gene is located on human Chromosome 2, loci q33-q34.
For Research Use Only. Not for Use in Diagnostic Procedures.
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