mRNA detection using flow

aleta knowles (a.knowles@vetp.usyd.edu.au)
Tue, 10 Dec 1996 10:42:43 +1100

Hi, we are new to the world of flow, cytokines and RNA and need some help.
We would like to look at cytokine mRNA levels in peripheral blood samples.
Has anyone out there used fluorescent labelled probes to detect mRNA levels
in peripheral blood for detection by flow cytometry (I assume it is
possible to probe for different mRNA concurrently using different fluoro
labels on probes??). Any references or hints would be greatly appreciated
(eg. are there detrimental effects from fixation, how to permeabilise, lots
of REALLY BASIC intructions required!!!).

Thanks
Aleta Knowles & Nicola Kingsford

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Aleta Knowles
Department of Veterinary Pathology
The University of Sydney Phone: 61 2 9351 7651
2006 NSW Fax: 61 2 9351 7348
Australia