>We are struggeling with intracellular staining of INFg/IL4 using BD
>FASTimmune cytokine system.
>If anyone has succeded -what is the trick?
One trick I recall hearing is that for the whole blood assay the peripheral
blood must be collected in heparin tubes not EDTA as the cytokine production
is a Ca+ dependent response. Good luck.
-------------------------------------
Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Cornell University Medical College
Manhasset, Long Island, New York
ph: 516-562-4641 fax: 516-562-2866
e-mail: thomasm@nshs.edu
Date: 12/10/96 Time: 15:40:21