I am looking for references and/or advice on the following:
Staining live, unfixed bacteria with Hoechst 33342 (or another DNA stain =
with similar spectral characteristics). I tried loading bacteria with H =
33342 but they did not take up the dye. Do I need to use EDTA or some =
other agent to get the H 33342 into the bacteria?
Does anyone have any tips or good references in general on sorting =
bacteria using a flow cytometer.
Thanks in advance,
Tom Knapp
Aurora Biosciences Corporation
11149 N. Torrey Pines Road
La Jolla, California 92037 USA
email "knappt@aurorabio.com"
Web Site "http://www.aurorabio.com"
(619) 452-5000 ex 125