I have used this technique with good signal amplification using
streptavidin labeled fluorescent microspheres. Once you can block the
non-specific binding by the microspheres they really provide a nice
signal. We presented a poster on the use of Molecular Probes's
streptavidin beads for signal amplification at ISAC in 1996.
You can also take a look at:
Biochim. Biophys. Acta 1983, 728, 112-20
Analytical Biochem. 1994, 222, 441-9
KUKURUGA@medmail.med.umich.edu wrote:
> I've heard of a technique for amplifying signal of low density
> antigens by
> creating layers of avidin-biotin complex, thereby increasing the
> available
> biotin targets for the finally added fluorescent-conjugated avidin.
> Anyone
> know about this, and do you have references? I've searched, but I
> guess
> I can't come up with the proper key words.
> Thanks.
> MAK.
>
> ---
> Mark A. KuKuruga, Managing Director
> University of Michigan Flow Cytometry
> kukuruga@medmail.med.umich.edu
-- -------------------------------------------------------- Mahesh K. Bhalgat, Ph.D. Staff Scientist and Flow Cytometry Products Manager Molecular Probes, Inc., OREGONPhone: 541-465-8300 Fax: 541-344-6504 Email: mahesh@probes.com
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