Re: Cryobiology applications
Iris A. Williams (iwilliam@hsc.usc.edu)
Fri, 19 Sep 1997 10:59:24 -0700 (PDT)
Dilute the cryoprotectants or remove cryoprotectants by pelleting the
cells and resupend cells in PBS or HepesPBS w/wo BSA(0.1%).
DMSO will kill cells and high osmolarity will cause major problems also.
Additives does cause FSC and SSC problems.
Iris