On Fri, 12 Sep 1997, adriano venditti wrote:
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> kcdol@samsung.co.kr wrote:
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> > Dear collegues
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> > I wonder how many laboratories do the nuclear TdT stain
> > by flow cytometric method after membrane permeabilization
> > not by cytospin followed by fluorscence microscopic reading?
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> > In case of flow cytometric method, which protocol for membrane
> > permeabilization you use?
> > Is the permeabilization reagent Triton-X, saponin, FACS Lysing Solution=
,
> > FACS Permeabilization Solution, Fix&Perm, or something special?
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> > And, have you ever tried to compare the results between
> > flow cytometric method and cytospin method?
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> > Any comments will be appreciated.
> > Thanks in advance.
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> > ---------------------------------
> > Chang-Seok Ki, M.D.
> > Dept. of Clinical Pathology
> > Samsung Medical Center
> > Seoul, Korea
> > Tel. 82-2-3410-2708
> > Fax. 82-2-3410-2719
> > E-mail. kcdol@samsung.co.kr
> > ---------------------------------
> We use to investigate nuclear Tdt expression by flow cytometry with a
> permeabilization protocol. We have used orthopermeafix and Perm and Fix
> and they compare well. A collegue of mine is currently developing a
> permeabilization protocol associating tryton and paraphormaldeyde with
> excellent results in terms of detection.
> Adriano Venditti
> Cattedra di Ematologia-Universit=E0 di Roma "Tor Vergata"
> tel.+39-6-59044101
> fax.+39-6-5915965
> E-mail.avenditti@pelagus.it
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