i've been following endocytosis (using dextran-labeled texas red) of a
glycoprotein (detected by FITC immunofluorescence) with confocal
microscopy. now i am interested in determining if the glycoprotein is
increasing the endocytic activity of my cell line. i think i will be able
to do this using a facscalibur (i.e., by looking for an increased
fluorescence intensity due to dextran uptake). the texas red isn't
compatible with the 488 line. can anyone suggest a conjugate that would be
appropriate (and not pH sensitive, as i do not want to lose signal in late
endosomes)? also, who, besides molecular probes, carries fluorescent
dextrans?
as always, i appreciate your help.
cheers,
barbara butcher
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Barbara A. Butcher, Ph.D.
James A. Baker Institute for Animal Health
Hungerford Hill Road
Cornell University
College of Veterinary Medicine
Ithaca, New York 14853
(607) 256-5647 Lab
(607) 256-5618 Office
(607) 256-5608 FAX
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