1. We have used two different CD14 Mabs on many AML4s with monocyte
differentiation : CD14 clone TUK4 and CD14 cloneMEM18. We find it very
seldom thet both of them are positive. The imunophenotype you give can very
well be consistent with a monocytic leukemis (M5a or b according to FAB).
2.CD5 is quite often "dim" in lymphoproliferative T cell disorders . Could
you characterize these cells further ? were they all CD4 or CD8 positive?
what about other T-cell markers as CD2,7 and CD3 membr. and cytoplasmic?
What about TCR gamma and delta?
Withe best wishes
Anna Porwit-MacDonald, MD, PhD
Heamatopathology Lab.
Karolinska Hospital Stockholm
>Cytometrists,
>I have two interesting cases that I would like some help with.
>1. The first patient appears to be a new acute leukemia with very
>large irregular cells. The bone marrow phenotypes CD13+,CD15+,CD33+,HLA DR+,
>CD45+(bright),CD14 My4+ but CD14 Mo2 negative.
>I would like to know others' experience with My4. Does it mark a
>more immature monocytoid cell? Would you call this myelomono-
>leukemia?
>2. The second case is a patient with normal numbers of all cell
>types in the peripheral blood but with two clearly different
>populations of CD5+ cells--one very bright and the other dimmer but
>still positive. They do not dual stain with B cell markers. In
>1993 the patient showed a T cell beta gene rearrangement. If we had
>not been aware of this we may not have worried so much about the
>staining. Has anyone noticed this pattern of CD5 reactivity?
>Thank you very much for your help.
>
>Michaeleen M. Collins
>
>