Re: Viability Question

Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
19 Aug 97 16:59:58 EDT

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Zip Kruger-Gray: "Hepatocytes by flow ..."
Previous message: Hoffmann, H. J.: "RNA amounts by flow"
Maybe in reply to: Dr Michelle Miller: "Viability Question"

In answer to Michelle Miller, FSC signals decrease when the cell membrane
becomes permeable to the external medium (thus altering the refractive index of
the cytoplasm). Trypan Blue enters the cell when the membrane becomes
permeable to Trypan Blue. Trypan Blue is larger than the small molecules (eg
water, NaCl, etc) of the medium, and for this reason a cell may appear dead
by FSC vs SSC before it appears dead by Trypan Blue. Since viability
indicators are all of different sizes, death becomes relative.....

Alice L. Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon, New Hampshire
NH 03756 USA
tel 603-650-7661
fax 603-650-6130
e-mail givan@dartmouth.edu

Next message: Zip Kruger-Gray: "Hepatocytes by flow ..."
Previous message: Hoffmann, H. J.: "RNA amounts by flow"
Maybe in reply to: Dr Michelle Miller: "Viability Question"