RNA amounts by flow

Hoffmann, H. J. (hjh@post1.tele.dk)
Tue, 19 Aug 1997 20:10:24 +0000

I would like to determine the amount of RNA in eosinophils by flow
cytometry to get an idea of the amount of RNA I can expect to extract
from the cells afterwards.

Does anyone have experience in using LDS 751 or a similar dye to
determine the content of RNA in cells.

All inspiring comments are welcome
Hans Juergen Hoffmann, Ph.D. - Clinical Scientist
Department of Respiratory Diseases
Aarhus University Hospital
Denmark
Tel +45 89 49 21 07 Fax +45 89 49 21 10