Quantitative detection of RNA by flow

Hoffmann, H. J. (hjh@post1.tele.dk)
Tue, 19 Aug 1997 09:37:54 +0000

I would like to determine the amount of RNA in eosinophils by flow
cytometry to get an idea of the amount of RNA I can expect to
extract from the cells afterwards.

Does anyone have experience in using LDS 751 or a similar dye to
determine the content of RNA in cells.

All inspiring comments are welcome

Hans J=FCrgen
Hans J=FCrgen Hoffmann - Lung Research Clinic
Aarhus University Hospital, Denmark