dear michaeleen
i dont know the difference in epitope specificity between the two CD14
clones, but if one is positive,and there is a positive (cytochemical)
esterase reaction there is certainly evidence for an "M5" variety monocytic
leukemia. Is there 'dim' CD4? that is another tip to monocytic
differentiation
>2. The second case is a patient with normal numbers of all cell
>types in the peripheral blood but with two clearly different
>populations of CD5+ cells--one very bright and the other dimmer but
>still positive. They do not dual stain with B cell markers. In
>1993 the patient showed a T cell beta gene rearrangement. If we had
>not been aware of this we may not have worried so much about the
>staining. Has anyone noticed this pattern of CD5 reactivity?
I am very familiar with this pattern. The dim CD5+ are most likely
CD3+CD8+, the bright are mostly CD3+CD4+.
regards
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Dr. Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Warren 525A
100 Blossom St
Massachusetts General Hospital
Boston MA 02114
v(617) 726-7481 fax(617) 724-3164