P. Echeagaray
Janet L. Jacobitz wrote:
>
> On Mon, 11 Aug 1997 17:20:23 -0700 (PDT) you wrote:
>
> In order to release most of the marrow cells from possible bone marrow
> particles we have for years used repeated aspiration of the aspirate through
> a small gauge needle (25 ga.). This seems to work fine, does not appear
> to damage the cells much and is fairly rapid. It is however not a very
> safe practice. I would like to stop having the people in the lab have to
> use these needles while manipulating the patient bone marrow in the lab.
> Does anyone have any better procedures for releasing cells in the
> particles?
> We use the frosted ends of microscope slides for tissues and this works
> fine but is impractical for aspirates.
>
> Tom McHugh
> Dept. Lab. Medicine
> UCSF
> mchugh@labmed.ucsf.edu
>
> We routinely use a sterilized fine mesh screen placed over a petri dish,
> pull the plunger out of a 12 ml disposable syringe, and use it to push the
> marrow through the screen. Use media to flush the screen. The screen can be
> purchased from Small Parts Inc and is called wire cloth type 304 ( or you
> may use a smaller mesh if desired).
> **********************************
> * Jan Jacobitz *
> * C-V Surgery Lab Manager *
> * Children's Memorial Hospital *
> * 2300 Children's Plaza Box 220 *
> * Chicago,IL 60614 *
> * phone: 773-880-4676 *
> * fax: 773-880-3088 *
> * e-mail:jjacobitz@nwu.edu *
> **********************************