At 03:31 PM 8/7/97 -0400, you wrote:
>
>Hi,
>
> I have an investigator who is analyzing murine embryonic fibroblasts
>(MEF) for cell cycle perturbations based on retrovirally infected cell cycle
>inhibitory proteins. We have regularly observed what appears to be a true
>tetraplod population, ie., exhibiting singlet cell properties based on pulse
>processing (FACScan FL-2A vs. FL-2W), PI nucelear isolation prep. We see
>this in both normal control and experimental preps.
> Is a tetraploid condition a known feature of MEF biology? These are
>isolated from fetuses and placed directly into primary culture. We have
>seen it often enough that I want to believe it, but it is controversial.
>Thanks for the input....regards, Dave G.
>Dave Gebhard
>Director FACS/ Oligonucelotide Synthesis Facilities
>Albert Einstein College of Medicine
>1300 Morris Park Avenue
>Bronx, New York 10461
>718-430-2724/ 3573
>
>
>
Dennis
*************************************************************************
* Dennis J. Young Voice : (619) 822-0407 *
* Flow Cytometry Core Facility FAX : (619) 822-0412 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
* http://www-core.ucsd.edu/flow-core.html *
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