I Would like to let folks know that I have brought the high background
in J774A.1 (FL2 peak channel of close to 200) to acceptable levels
(P.Ch.of around 5)and still have the ability to detect my positive
control marker. The main culprit seemed to be FL2 spill over into FL1
and FL3 which I was able to compensate for. A change to media w/out
phenol red seemed to help as well. Thanks again for the helpful
suggestions.
Chris Reed
Senior in Biology
Washington State University