Try contacting the ATCC...sorry, don't have the address, but they are a
bank of thousands of cell lines from which you can purchase.
(A'flowin' just down the road from you!......)
P. Echeagaray
Flow Cytometry Services
Southern Research Frederick
Frederick MD
echeagaray@sri.org
Theresa L. Fecik wrote:
>
> Hi all,
>
> I'm a newbie here on this board--though, not necessarily a newbie in flow
> cytometry. I have about 10 years experience in flow--but, I have also
> been working in other areas of the lab at the same time, i.e.,
> hematology, coagulation, urinalysis, and special hematology testing. So,
> I guess I'm more a jack of all trades, rather than a master of this one.
> That is why I'm asking such a basic question.
>
> When I worked at the Johns Hopkins Oncology Center, and did flow
> cytometric analyses of leukemias, we used leukemia cell lines (KG1a,
> Daudi, and MOLT3 cells) as positive controls for our leukemia antibodies.
> The cells were supplied to us by one of the oncology research labs.
> Now I work at a community hospital that has a flow cytometry lab, and I
> no longer have access to these cell lines. Does anybody know if there
> are stabilized leukemia cell lines that are sold commercially? As you
> can see, they don't let me out of my cage too
> often.......................
>
> Thanks very much in advance for any information you can give me.
>
> Going with the flow,
> Terry Fecik
> Sinai Hospital of Baltimore