Re[2]: Autofluoorescence problems (sic)

Dennis_Young@CIS.ucsd.edu
Thu, 31 Jul 1997 13:25:00 -0700

As this journal is not available in our extensive library system at the
University of California, could you perhaps hint at the protocol used?

I have the Cytometry 8:23-239 (1987) paper by Beisker, Dolbeare and Gray,
(and the Carnegie,J.A.,et al Journal of Histochemistry and
Cytochemistry:244 pp308-310, 1980) but have been afraid to try the
borohydride. Sigma tech support wasn't very encouraging.

How long is the NaBH4 stable in PBS?

Dennis
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______________________________ Reply Separator _________________________________
Subject: Re: Autofluoorescence problems
Author: Karenrt@AOL.COM at @UCSD
Date: 7/27/97 4:47 PM

There is a very recent article on this subject in the latest issue of Communic
ations in Clinical Cytometry:

Mosiman VL, Patterson BK, Canterero L, Goolsby CL. Reducing cellular
autofluorescence in flow cytometry: an in situ method. Communications in
Clinical Cytometry, Vol 30, No 3, 15 June 97, 151-156.

Hope this helps!

Karen Tamul, MS, MT(ASCP)SI
Mid-Atlantic Sales Representative
Caltag Laboratories
Phone/Fax: (301) 540-3712
E-mail: karenrt@aol.com

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