http://nucleus.immunol.washington.edu/Research_facilities/Apps/7aad.html
Dave Coder
dcoder@u.washington.edu
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From: pathol-immuno biol-ther <biol-ther.pathol-immuno@psl.ap-hop-paris.fr>
To: cyto-inbox
Subject: PI gating on FL3
Date: Wednesday, July 30, 1997 3:09 AM
Dear all,
I need your comments to settle a discussion in the lab regarding PI-based
gating of viable
cells in FL3. I do membrane staining with a combination of FITC and PE
conjugated
Moabs, then I stain cels with PI, make exclusion gate on PI-positive cells
in FL3 and then
evaluate FL1/FL2 staing on PI-negative cells. Is that a correct approach
and if not what are
other ways?
Thank you,
Karolina Palucka