PI gating on FL3

pathol-immuno biol-ther (biol-ther.pathol-immuno@psl.ap-hop-paris.fr)
Wed, 30 Jul 1997 10:09:49 +0000

Dear all,
I need your comments to settle a discussion in the lab regarding PI-based gating of viable
cells in FL3. I do membrane staining with a combination of FITC and PE conjugated
Moabs, then I stain cels with PI, make exclusion gate on PI-positive cells in FL3 and then
evaluate FL1/FL2 staing on PI-negative cells. Is that a correct approach and if not what are
other ways?
Thank you,
Karolina Palucka