Many thanks to those who responded re my laser troubles: we had a bad
15VDC power supply, and once I swapped that we were back in business.
Now I am faced with researchers inquiring about the feasibility
of two different projects, and I can't find much in the literature.
We're still looking, but any first-hand experiences or tips would be
greatly appreciated--I'm not sure either of these are feasible:
a) Subject A wants to hybridize oligos to specific RNA sequences, to
detect levels of expression of specific genes. I'm aware of methods for
measuring mRNA content by flow, but not of specific genes. I have
feeling our signal would be verrry dim.
b) Subject B has already collected (! arrgghh !) spleen and bone
marrow samples from mice exposed to high levels of radiation, and
would like to do micronuclei assays or chromatin fragmentation. The
Nusse et al chapter in the "D.R.C., Methods in Cell Bio. vol II" seems
promising, but only with cell cultures. Primary tissue apparently has
a lot of debris, which confuses the detection of micronuclei. I would
also think that this will be especially difficult with heterogeneous
tissue like bone marrow.
Anyone have any encouraging words....anyone?
Thanks in advance,
Steve
PS--I saw the message from Peter, but has anyone heard from the folks at
CSU? Best wishes and my sympathy to everyone in Fort Collins!
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Steve G. Hilliard Linux: In a world
Cell Analysis Facility without fences,
University of Georgia who needs Gates?
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