Re: GFP/Apoptosis

Mario Roederer (Roederer@Beadle.Stanford.EDU)
Tue, 29 Jul 97 09:12:47 -0700

> Has anyone tried this combination? Or are there any other
> apoptosis markers that would work with GFP?

We haven't specifically tried GFP with annexin, but there is no reason for this
not to work--we often use GFP with a wide variety of fluorochromes, like PE,
Cy5PE, TR, APC, etc; annexin V can be conjugated to any of these to be used in
combination with GFP. If you have the annexin V protein (it can be obtained
both commercially and in collaboration with some scientists producing
recombinant versions), conjugation instructions can be found at
"http://cmgm.stanford.edu/~roederer/abcon/".

BTW, the annexin V staining survives 0.5% paraformaldehyde fixation just fine.

mr