intracellular antigens/PI

Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
28 Nov 95 13:18:32 EST

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Hello Flowers,
For those of you following Clevenger-type methods for staining cells for DNA
plus an intracellular antigen: after p-formaldehyde fixation and treatment
with 0.1% Triton-X, do you leave detergent in the washing and staining
solutions for all subsequent steps? Published protocols seem rather
ambiguous on this point. We want to cut down on non-specific staining, but
suspect that the triton-X will interfere with our specific staining as well.
Thanks for your help.
Alice Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon, NH 03756, USA
tel: 603-650-7907
fax: 603-650-6130

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