PI TOXICITY?

Immuno Flow (Immuno_Flow_at_APPLIC1@ccmail.llu.edu)
Tue, 28 Nov 95 12:29:23 PST

Please help me win a bet with my boss (I already owe him 3 lunches).
Since the latest discussion about PI staining, we were discussing
protocols and there were a few shall we say disagreements...

1) Is PI toxic to cells?

2) What is the minimum incubation time for PI and cells, say for
live/dead discrimination or DNA?

Thanks (I hope),

Judy the Gambler

The Immunology Center
Loma Linda University Medical Center
11234 Anderson Street, Rm 2573
Loma Linda, CA 92354

(909) 824-0800 Ext. 45376