TdT positive cell lines
Tom Mchugh (mchugh@pangloss.ucsf.EDU)
Mon, 23 Oct 1995 15:58:29 -0700 (PDT)
We currently use the cell line RPMI 8402 as a TdT positive control both
in flow and on cytopreps (we use RAJI as a negative line). Our cultured
cells are losing their TdT and the new aliquot we purchased from the
Coriell Mutant Cell Repository is TdT negative by flow and on cytoprep
(by monoclonal and polyclonal antibody). Does anyone know which
commercial cell line is good as a positive TdT control? I would rather
not buy many to test just to find out which is best.