Re: Intracellular cytokines

/G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Mon, 11 Sep 1995 06:33:00 -0400

Try to contact:
Dr. C.T.Nebe
Klinikum Mannheim
University of Heidelberg
Tel.:+49-383/2222
Fax:+49-621-383/3819

I remember when he edited a German book on Clinical Methods
in Flow Cytometry (subsequently published by Schmitz et al)
that they had a routine protocol for heparinised whole blood
called:

Detection of intracellular cytokine
- functional characterisation of immunecompetent cells in
immune defects
- monitoring of immunosuppressive therapy

from a group in Bochum. They used a system of isotype
specific secondary antibodies and triple colour
fluorescence.


Gerhard Nebe-v.Caron
Unilever Research, Colworth Laboratory Sharnbrook,
Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
E.mail: gerhard.nebe-von-caron@urcgb.sprint.com



______________________________ Reply Separator _________________________________
Subject: Intracellular cytokines
Author: DONNAL@novell1.dept-med.pitt.edu at INTERNET
Date: 08/09/95 20:37

Two questions about detection of intracellular cytokines:

1) Does anyone have an opinion concerning the relative merits of
Brefeldin A and monensin for blocking cytokine secretion?

2) Does anyone have information on the availability (commercial or
otherwise) of directly conjugated anti-cytokine antibodies. We have
only been able to come up with one (FITC anti-IL2 from Biosource).

Thanks,

Albert D Donnenberg
University of Pittsburgh
Albert D. Donnenberg, PhD
Associate Professor of Medicine