surface staining macrophages

brett (brett@borcim.wustl.edu)
Mon, 24 Jul 1995 20:49:04 -0600

Hello. I'd like to look at expression of a surface protein on mouse macrophage-
like cell lines by flow cytometry. I'm worried about decorating them with our
mouse mAb, which could be bound by FcR's on these cells. Two options I thought
of were 1) absorb mouse myeloma proteins to the FcR's, and FITC-label by mAb;
2) cleave the mAb into Fab or F(ab')2 fragments which could be FITC-conjugated.

What have other people done in these circumstances? What works and what doesn't?

Thanks in advance,

Brett Lindenbach

Program in Immunology
Washington University - St Louis
brett@borcim.wustl.edu