Re BU-1 clone

ZBIGNIEW DARZYNKIEWICZ (darzynk@nymc.edu)
Tue, 25 Jul 1995 09:03:09 -0500

To the ongoing discussion on a possibility of simultaneous analysis
of BrdUrd incorporation and cell immunophenotype let me add the
following suggestion: the SBIP method of analysis of BrdUrd
incorporation (See June issue of Cytometry and Cancer Res., 54:4289,
1994) does not require DNA denaturation and is compatible with
surface immunophenotyping. Brief fixation with formaldehyde
stabilizes e.g. FITC labeled antibody, the cells are then briefly
illuminated with UV light, permeabilized and DNA strand breaks
labeled with digoxygenin or biotin conjugated dUTP. The later are
detected with antibody or avidin conjugated fluorochrome of another
color than FITC. Cell morphology is well preserved.
Zbigniew Darzynkiewicz