I have an investigator who wants to look at intracellular distribution of a
drug using confocal laser scanning microscopy. The drug is not fluorescent,
so he has a monoclonal antibody (FITC conjugate) to the drug. Our question
is, during permeablization to allow the antibody into the cells, what is the
best way to prevent the drug from leaching out of the cells? I suppose a
pre-permeablization paraformaldehyde fixation step would be helpful, but I
would like to hear from anyone who has done such drug studies and knows if
this is an effective approach.
Thanks in advance.