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Michelle N. Fiordalisi, Ph.D.
Clinical Immunology Fellow
University of North Carolina Hospitals
email: mnfiord@med.unc.edu
Phone: 919-966-4058 FAX: 919-966-0486
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---------- Forwarded message ----------
Date: Mon, 21 Apr 1997 08:52:52 -0400
From: Stacie Anderson <staciea@nhgri.nih.gov>
To: cyto-inbox
Subject: Re: Intracellular cytokines in monocytes
We have just started using the PharMingen kit - "CytoStain" - to
look at IL-12 production in monos/macrophages. The kit is easy to use and
gives nice results - just remember to keep your cells in the Perm/wash
solution even during antibody staining! We are using it for clinical
samples also. You can also buy this kit with brefeldin or monensin to see
which works best in your system. Hope this is helpful. Stacie Anderson
Stacie Anderson
Laboratory of Gene Transfer, Hematopoeisis Section
NCHGR, NIH
Room 2C12
49 Convent Drive
Bethesda, MD 20892-4442