Re: Intracellular cytokines in monocytes (fwd)

Michelle N Fiordalisi (mnfiord@med.unc.edu)
Thu, 24 Apr 1997 12:00:37 -0400 (EDT)

---------- Forwarded message ----------
Date: Wed, 23 Apr 1997 10:44:55 -0400
From: Stacie Anderson <staciea@nhgri.nih.gov>
To: cyto-inbox
Subject: Re: Intracellular cytokines in monocytes

We use CD14 as our monocytic marker and it does not seem to be
affected by the stimulation although we have not specifically stained the
cells pre and post stimulation. We had a monocyte product from apheresis
and the staining was as we would have expected. We did see a difference in
IL-12 production depending on the stimulation that the cells received.
Good luck! Stacie

Stacie Anderson
Laboratory of Gene Transfer, Hematopoeisis Section
NCHGR, NIH
Room 2C12
49 Convent Drive
Bethesda, MD 20892-4442