Isotype Concentrations

Tim Holzer (HOLZERT@centocor.com)
Tue, 22 Apr 1997 15:21:25 -0400

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: LAADAMS@am.pnu.com: "Re: apoptosis"
Previous message: Peter Nagy: "internalization of antibodies"

If I have a mixture of 3 unlabeled mouse monoclonal Abs to different cytokeratins, how do
I choose an isotype control concentration? Each individual Mab is at 1 microgram/ml
(total of 3 micrograms/ml) and each is an IgG1 isotype. Would you suggest I use a single
unlabeled IgG1 control at 1 or at 3 micrograms/ml? I also want to use these for some
immunohistochemistry staining. Can I use the same isotype control for this?
Thanks for any comments!

Tim Holzer
Centocor, Inc.
holzert@centocor.com
610-889-4492

Next message: LAADAMS@am.pnu.com: "Re: apoptosis"
Previous message: Peter Nagy: "internalization of antibodies"