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> From: Wallace Lauzon <wlauzon@uottawa.ca>
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: A GOOD monocyte marker?
> Date: Monday, March 10, 1997 3:11 PM
>
>
> Hi,
>
> I have a rather simple sounding question, particularly for the sorters
> out there. I am looking to positively separate CD4, CD8, and monocytes
> from Ficoll-hypaque separated blood samples. I intend to use avidin
> columns and biotinylated antibodies. Obviously CD4 and CD8 will be use
> for the isolation of these lymphocytes, but the best monocyted marker is
> less clear. I have considered CD14, CD15, and CD33. My goal is to
> obtain a pure monocyte sample, not necessarily the entire monocyte
> population. The cells will be fixed immediately after they are
> isolated and the subsequent manipulations are not affected by the
> activation state of the cells. Does anyone have experience with these
> surface markers or others that would be more appropriate for isolating a
> relatively pure monocyte population?
>
> Thanks in advance
>
> Wallace Lauzon
> Dept. of Microbiology and Immunology
> University of Ottawa
>