Re: CD45 Negative PBL

Timothy J Farley (tfarley@computer.net)
Mon, 10 Mar 1997 19:47:46 -0500

Mark, as Dennis Young noted, the question is are the CD45- events
lymphocytes or even leukocytes? I've heard that giant platelets or
platelet aggregates can end up in standard lymph gates. I've seen this
problem exacerbated in apheresis collections older than 24 hours.
Tim Farley, New York Blood Center

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> From: Mark Rehse <markrehse@CellPro.com>
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: CD45 Negative PBL
> Date: Thursday, March 06, 1997 12:42 PM
>
>
>
> Dear Flow Community,
> We use CD45 FITC (Immunotech clone J33) in our two color
> immunophenotyping panels to identify the WBC component in apheresis
> and buffy coat and eliminate RBC's, debris and dim staining platelets
> from subsequent enumeration of T and B cells, stem cells, NK cells,
> monocytes and granulocytes using PE-conjugated antibodies. This
> method is superior to traditional light scatter gating, however, there is
a
> population of CD45- cells which are equivalent in size and granularity to
> T and B cells which we have not yet been able to identify in our panels.
> Does anyone have experience with this population? Is it a problem with
> this clone?
> Thanks in advance.
> MarkRehse@CellPro.com