My colleague and I would like to set up a test for the expression of
cytoplasmic IgM for differential diagnosis of B-ALL and would
appreciate any comments /advices /references on the problems that we
encountered, mainly how would one tell the difference between a surface IgM
versus a cytoplasmic IgM? Can I somehow block the staining of surface IgM
or can I quench the surface staining as is being done with phagocytosis
tests? And is there any good controls for the tests?
We are also evaluating the possibility of performing a cytoplamic CD3
staining for diagnosis of T-ALL and, quiet possibly use it for monitoring
of minimal residual disease. We have the same concerns for this
test as we mentioned for cytoplasmic c-mu and would appreciate any comments
/advices /references.
Warm regards,
Austin Lin
Core Facility Laboratory-Flow Cytometer,
Cheng Gung Medical College
Tainan, Taiwan