Re: Live vs. Dead cells?

Dave Coder (dave@nucleus.immunol.washington.edu)
Fri, 24 Jan 97 08:12:33 -0800

Try 7-AAD (7-amino-actinomycin D). Protocol and illustrations =
(including important fluorescence overlap compensation details) are on =
the Web at:

URL =
<http://nucleus.immunol.washington.edu/Research_facilities/Apps/7aad.html=
>

Dave Coder
dcoder@u.washington.edu

Begin forwarded message:

Date: Thu, 23 Jan 97 09:30:44 EST
From: "Adrian Moris" <Adrian_Moris@sratech.com>
Encoding: 11 Text
To: cyto-inbox
Subject: Live vs. Dead cells?

Dear Flow Folks:
=20
A Co-worker and I are are wondering if there is a way to stain =
cells=20
for flow which will distinguish between live and dead cells when=20=

co-stained with a PE conjugated Ab. Does anyone have such a =
protocol=20
they would share?
=20
Thanks
=20
Adrian Moris
SRA Technologies