Re: murine stem cells

Rochelle A. Diamond (diamond@cco.caltech.edu)
Wed, 15 Jan 1997 17:13:44 -0800 (PST)

Arnold-
Lin neg is indeed a cocktail of antibodies which are markers for mature
hematopoeitic lineage cells. We use cocktails of them which are
biotinylated so that they can all be marked by one color like fitc or pe
depending on the choice for your stem cell markers.

What kind of stem cells will you be sorting? Bone marrow, fetal liver? The
panel might be different depending on what you are after also- myeloid,
lymphoid etc.

Check out Morrison et al from the Hertzenberg group at Stanford. I can
look up the reference tomorrow.

Rochelle Diamond
Caltech Flow Cytometry/Cell Sorting Facility
On Wed, 15 Jan 1997, Arnold Mixon wrote:

>
> An investigator read somewhere(?) of a panel of monoclonal Abs used
> to identify murine progenitor/stem cells. Of interest is an antibody called
> "lin-neg." Anyone out there heard of it before/know of it's source? It
> sounds like it might be a cocktail of different Abs.
> Thanks,in advanced, to all responses.
>
> Arnold
> Arnold Mixon
> National Institutes of Health
> N.C.I., Surgery Branch
> Flow Cytometry Lab
> Tel.# 301-496-9816
> E-mail: Arnold_Mixon@nih.gov
>
> "Just the FACS Ma'am - I only want the FACS !!"
>
>