Calcium Flux Experiment

Marcia Woda

In this experiment, rat T cells were loaded with the green Calcium indicator Fluo-3 . Dissolution of the dye in aqueous solution was facilitated by the use of Pluronic f-127, a non-ionic detergent. A modest calcium flux was noted in only one of three animals in response to the cytokine, Rantes. Optimal loading and maximum Ca2+ flux was verified by the addition of a calcium ionophore , Ionomycin, seen in the lower right dotplot

Back to cell function assays

Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web, EMAIL