Purdue Cytometry CD-ROM Volume 3
DNA analysis Section advisor:
Dr. William Telford.

Measurement of cellular DNA content and especially analysis of the cell division cycle have become among the most popular applications of flow cytometry. Potential uses include the determination of ploidy (often with significant implications for such areas as clinical tumour prognosis), analysis of cell division kinetics (e.g. S-phase fraction, doubling time, mitotic index, etc.), DNA Index measurement, flow karyotyping, and flow sorting of individual chromosomes, to mention but a few. However, such measurements often can be problematic and do require strict quality control. It is hoped that the material included in this section may serve to assist readers who might and who do work in this important field of flow cytometry.

  1. Multiparametric flow cytometry for cell proliferation studies.   G. Mazzini, et al.
  2. Multiparametric flow cytometry for determining DNA content in whole cells.   M. Carbonari, et al.
  3. Combined measurement of apoptosis (phosphorylation) and cell cycle.   G. Basso
  4. Detection of aneuploidy using propidium iodide  M. Woda
  5. The effect of DNA damaging agents on cell cycle.  M. Woda
  6. DNA content in yeast.  M. Woda
  7. Example data files showing DNA analysis by flow cytometry.  H. S. Kruger Gray, et al.
  8. Selected email discussion of apoptosis
  9. Selected email discussion of DNA analysis

Note: These data have been provided by colleagues worldwide. Appropriate acknowledgement should be made when material is used.

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu