Detection of Aneuploidy Using Propidium Iodide

Marcia Woda

Cells with altered DNA content are identified by staining alcohol-fixed, RNAase treated cells with the intercalating dye, PI. The first peak on the left represents normal diploid cells. The next major peak represents tumor cells with increased DNA content and DNA index of 1.58. Combined with an additional fluorescent antibody specific for a unique cell population, this technique is even more powerful and allows the more accurate S phase analysis of muliple overlapping populations.

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web, EMAIL