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John,
There are three references you should probably take a look at if you
haven't already:
Dickson et al. 1992, Nuclear DNA content variation within the Rosaceae.
American Journal of Botany. 79:1081-1086. They used the protocol found in:
Arumuganathum and Earle. 1991. Estimation of nuclear DNA contents of
plants by flow cytometry. Plant Molecular Biology Reporter. 9:229-233.
This protocol uses PI (plus RNase). The third referece uses H0: Moyne et
al. 1993. Relationship between cell ploidy and regeneration capacity of
long term Rosa hybrida cultures. Plant Science. 93:159-168.
Good luck!
Wendy
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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