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For a method for flow detection of cytokine mRNA, try Morvan, PY et al (1994)
"In situ hybridisation and cytofluometric analysis of cytokine mRNA during in vitro
activation of human T cells," European Cytokine Network 5 (5): 469- 480.
I'm interested in this too, but probably won't have a chance to try it for a couple of
months - please let me know how you get on! If it worked well it would be a lot
cheaper (and more versatile) than using MoAb's.
Lachy McLean
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Lachy McLean
Senior Lecturer in Clinical Immunology
Department of Molecular Medicine, University of Auckland
Private Bag 92019, Auckland 1, New Zealand
Tel: 64-9-373-7599 ext 6170 Fax 64-9-373-7492
EMail: l.mclean@auckland.ac.nz
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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