 |
 |
 |
 |
 |
>
> Hello,
> I need to measure the proliferation of a specific cell population
> (macrophages) and think that one of the bromo-deoxy-uridine and antibody
> kits would do the job.
>
> Does anyone have any recommendations about which manufacturer's kit does a
> good job? I have experienced variable quality in purchased kits, and
> would like to hear from someone who has actually used these before
> investing the money. Thanks!
>
> Rosemarie
>
Dear Rosemarie,
you don't need a kit for that. I use 10 mM BrdU from SIGMA in the
cell media and then the monoclonal BrdU antibody from Boehringer
Mannheim to do a PI-BrdU doublestaining. It works very nice and their
protocol is quite simple.
Ioakim Spyridopoulos, MD
Dept. of Cardiovascular Research
Boston, TUFTS University
email: ioakim@user1.channel1.com>
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web