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We have a investigator who is doing research with transgenic mice. He
also had high background problems. We found that the fixative was
causing all the background. So we aways used fresh cells, unfixed
and did a titer of the monoclonal to obtain optimal staining
characteristics.
Hope this helps,
Davin
Davin Jutila
Research Imaging/Flow Cytometry Facility
Southern Illinois University School of Medicine
Springfield, IL 62794-1220
email: djutila@wpsmtp.siumed.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web