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Eric P Miller
Edinburgh Medical Oncology Unit
"I am Pentium of Borg. Division is futile......you will be approximated."
Star Trek - Generations. Two Captains, one density.
On Thu, 25 Jan 1996, Gary Durack wrote:
> This is a post from a colleague ...
>
> Cytometry Experts,
>
> We are doing flowcytometric analysis of nuclear DNA content and ploidity
> determination of paraffin embedded archival pathological lymphoma cases in
> dogs.We dewax the 30um sections by two changes of xyleneand dehydrate
> with sequential ethanol concentrations and distilled water.Finally we
> digest the tissue by pepsin and stain by standard PI staining method.
> We get nice diploid and aneuploid peaks,however the fluorescence of the
> deparaffinized samples is lower than in the fresh material.
>
> Questions:Is this the expected pattern of paraffin embedded tissue?
> If yes:what is the explaination?
> If not:how can we increase PI uptake in archival lymphoid tissues?
>
> farkas@ux1.cso.uiuc.edu
>
> Amnon Farkas
> Univeristy of Illinois
> School of Veterinary Medicine
>
> Gary Durack
> University of Illinois Biotechnology Center
> Flow Cytometry Facility
>
> Voice (217) 244-0559
> FAX (217) 244-0466
>
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web